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KMID : 0384119930130020271
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 2 p.271 ~ p.274
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Evaluation of Aniline Blue Dye Method for Rapid Identification of Candida albicans
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Abstract
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Candidiasis and the other serious candidial infections are the most frequently encountered opportunistic fungal infections and the incidence of it is increasing now. Candida albicans infections occur in a substantial number of patients with
immunocompromised, immunocompetent, and aging hosts in the population. For these reasons, the ability to rapidly isolate and identify C. albicans, become ever mere important. We studied the ability of YM agar containing 0.01% aniline blue dye
(YMAB)_ to
identify C. albicans. This method was discovered by Fung et al. We tested 79 clinical strains identified as C. albicans and 42 other strains of yeast. Organism inoculated YMAB plate were incubated for 12-24 hours at 30℃, and colonies were
observed
for
yellow-green fluorescence under long-wave UV light. All of the C. albicans fluoresced except two and only one strain of C. parapsilosis fluoresced. The sensitivity, specificity, and test efficiency were 97.5%, 97.6%, and 97.5%, respectively.
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KEYWORD
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